human hcc cell lines sk hep1 (ATCC)

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ATCC human hcc cell lines sk hep1

TRNP1 knockdown affects the proliferation, invasion, migration and apoptosis of HCC. (A) The protein expression of TRNP1 in the HCC cell lines. (B) Images (left panel) and quantified analysis (right panel) of TRNP1 expression detected by western blot <t>in</t> <t>Sk-hep1</t> and YY8103 cells. (C) Cell proliferation of TRNP1 -NC, TRNP1 -sh1, and TRNP1 -sh2 groups in Sk-hep1 and YY8103 cells was evaluated by CCK-8 assay. (D) Images (left panel) and quantified analysis (right panel) of clone formation assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (E,F) Images (left panel) and quantified analysis (right panel) of transwell migration and invasion assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. Scale bars: 100 µm. (G) Flow cytometric assays detecting the effects of TRNP1 knockdown on apoptosis in HCC cells (left panel) and statistical results (right panel). (H) Images (left panel) and quantified analysis (right panel) of apoptosis-related proteins expression detected by western blot in Sk-hep1 and YY8103 cells. (I) Expression of stem cell genes in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; NC, negative control; TRNP1 , TMF1-regulated nuclear protein 1.

Human Hcc Cell Lines Sk Hep1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hcc+cell+lines+sk+hep1/SK-HEP-1/pmc13187988-53-4-16
Average 97 stars, based on 1619 article reviews

human hcc cell lines sk hep1 - by Bioz Stars, 2026-09

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1) Product Images from "TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma"

Article Title: TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma

Journal: Journal of Gastrointestinal Oncology

doi: 10.21037/jgo-2025-1-1084

Figure Legend Snippet: TRNP1 knockdown affects the proliferation, invasion, migration and apoptosis of HCC. (A) The protein expression of TRNP1 in the HCC cell lines. (B) Images (left panel) and quantified analysis (right panel) of TRNP1 expression detected by western blot in Sk-hep1 and YY8103 cells. (C) Cell proliferation of TRNP1 -NC, TRNP1 -sh1, and TRNP1 -sh2 groups in Sk-hep1 and YY8103 cells was evaluated by CCK-8 assay. (D) Images (left panel) and quantified analysis (right panel) of clone formation assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (E,F) Images (left panel) and quantified analysis (right panel) of transwell migration and invasion assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. Scale bars: 100 µm. (G) Flow cytometric assays detecting the effects of TRNP1 knockdown on apoptosis in HCC cells (left panel) and statistical results (right panel). (H) Images (left panel) and quantified analysis (right panel) of apoptosis-related proteins expression detected by western blot in Sk-hep1 and YY8103 cells. (I) Expression of stem cell genes in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; NC, negative control; TRNP1 , TMF1-regulated nuclear protein 1.

Techniques Used: Knockdown, Migration, Expressing, Western Blot, CCK-8 Assay, Staining, Cell Counting, Negative Control

TRNP1 promotes HCC proliferation in vivo . The representative images (A), tumor weights (B) and tumor growth curves (C) of xenograft tumors from Sk-hep1 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 cells with/without TRNP1 knockdown. (E) Representative images (left panel) and quantitative analysis (right panel) of IHC staining of PCNA in xenografts of Sk-hep1 cells with TRNP1 -NC, and TRNP1 -sh2. Scale bars: 50 µm. *, P<0.05; **, P<0.01. HCC, hepatocellular carcinoma; IHC, immunohistochemistry; NC, negative control; PCNA, proliferating cell nuclear antigen; TRNP1 , TMF1-regulated nuclear protein 1.

Figure Legend Snippet: TRNP1 promotes HCC proliferation in vivo . The representative images (A), tumor weights (B) and tumor growth curves (C) of xenograft tumors from Sk-hep1 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 cells with/without TRNP1 knockdown. (E) Representative images (left panel) and quantitative analysis (right panel) of IHC staining of PCNA in xenografts of Sk-hep1 cells with TRNP1 -NC, and TRNP1 -sh2. Scale bars: 50 µm. *, P<0.05; **, P<0.01. HCC, hepatocellular carcinoma; IHC, immunohistochemistry; NC, negative control; PCNA, proliferating cell nuclear antigen; TRNP1 , TMF1-regulated nuclear protein 1.

Techniques Used: In Vivo, Western Blot, Knockdown, Immunohistochemistry, Negative Control

TRNP1 affects the malignant phenotype by targeting the c-Kit/STAT3 signaling pathway. (A) Volcano plot of differentially expressed genes in Sk-hep1 cells with/without TRNP1 knockdown. (B) KEGG enrichment analysis of differentially expressed genes. (C) Expression of relative KIT mRNA in Sk-hep1 cells with/without TRNP1 knockdown. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. (E) Images (left panel) and quantified analysis (right panel) of EMT markers (E-cadherin, N-cadherin) detected by western blot in Sk-hep1 and YY8103 cells. (F,G) Representative images (left) and quantitative analysis (right) of IHC staining of c-Kit, P-STAT3 and E-cadherin, N-cadherin in xenografts of Sk-hep1 cells with/without TRNP1 knockdown. Scale bars: 50 µm. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. EMT, epithelial-mesenchymal transition; FC, fold change; FDR, false discovery rate; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; KEGG, Kyoto Encyclopedia of Genes and Genomes; mRNA, messenger RNA; NC, negative control; STAT3, signal transducers and activators of transcription 3; TRNP1 , TMF1-regulated nuclear protein 1.

Figure Legend Snippet: TRNP1 affects the malignant phenotype by targeting the c-Kit/STAT3 signaling pathway. (A) Volcano plot of differentially expressed genes in Sk-hep1 cells with/without TRNP1 knockdown. (B) KEGG enrichment analysis of differentially expressed genes. (C) Expression of relative KIT mRNA in Sk-hep1 cells with/without TRNP1 knockdown. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. (E) Images (left panel) and quantified analysis (right panel) of EMT markers (E-cadherin, N-cadherin) detected by western blot in Sk-hep1 and YY8103 cells. (F,G) Representative images (left) and quantitative analysis (right) of IHC staining of c-Kit, P-STAT3 and E-cadherin, N-cadherin in xenografts of Sk-hep1 cells with/without TRNP1 knockdown. Scale bars: 50 µm. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. EMT, epithelial-mesenchymal transition; FC, fold change; FDR, false discovery rate; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; KEGG, Kyoto Encyclopedia of Genes and Genomes; mRNA, messenger RNA; NC, negative control; STAT3, signal transducers and activators of transcription 3; TRNP1 , TMF1-regulated nuclear protein 1.

Techniques Used: Knockdown, Expressing, Western Blot, Immunohistochemistry, Negative Control

TRNP1 knockdown enhances the therapeutic response of HCC to PD-1 blockade. (A) The correlation between the gene expression of TRNP1 and PDCD1 in human HCC samples using the GEPIA web tool. (B) Western blot images (left) and quantitative analysis (right) of TRNP1 knockdown in Hepa 1-6 cells are shown below. The representative images (C), tumor weight (D) and tumor growth (E) of xenograft tumors from Hepa 1-6 cells with NC and sh TRNP1 with/without anti-PD-1. (F) Lollipop graph illustrates the correlation between immune infiltration and TRNP1 expression. (G) The proportion of M1 and M2 macrophages in NC and TRNP1 knockdown Hepa 1–6 subcutaneous tumors was detected by flow cytometry, along with statistical data. (H) Volcano plot of ANXA1 expression in Sk-hep1 cells with or without TRNP1 knockdown. q-PCR (I) and ELISA (J) analyses show the expression of ANXA1 in mouse subcutaneous tumor tissues. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. ANXA1, annexin A1; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GEPIA, Gene Expression Profiling Interactive Analysis; HCC, hepatocellular carcinoma; NC, negative control; PD-1, programmed cell death protein 1; q-PCR, quantitative real-time polymerase chain reaction; TRNP1 , TMF1-regulated nuclear protein 1.

Figure Legend Snippet: TRNP1 knockdown enhances the therapeutic response of HCC to PD-1 blockade. (A) The correlation between the gene expression of TRNP1 and PDCD1 in human HCC samples using the GEPIA web tool. (B) Western blot images (left) and quantitative analysis (right) of TRNP1 knockdown in Hepa 1-6 cells are shown below. The representative images (C), tumor weight (D) and tumor growth (E) of xenograft tumors from Hepa 1-6 cells with NC and sh TRNP1 with/without anti-PD-1. (F) Lollipop graph illustrates the correlation between immune infiltration and TRNP1 expression. (G) The proportion of M1 and M2 macrophages in NC and TRNP1 knockdown Hepa 1–6 subcutaneous tumors was detected by flow cytometry, along with statistical data. (H) Volcano plot of ANXA1 expression in Sk-hep1 cells with or without TRNP1 knockdown. q-PCR (I) and ELISA (J) analyses show the expression of ANXA1 in mouse subcutaneous tumor tissues. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. ANXA1, annexin A1; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GEPIA, Gene Expression Profiling Interactive Analysis; HCC, hepatocellular carcinoma; NC, negative control; PD-1, programmed cell death protein 1; q-PCR, quantitative real-time polymerase chain reaction; TRNP1 , TMF1-regulated nuclear protein 1.

Techniques Used: Knockdown, Clinical Proteomics, Gene Expression, Western Blot, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Negative Control, Real-time Polymerase Chain Reaction

Knockdown:

Article Title: Genomic Analysis of Vascular Invasion in HCC Reveals Molecular Drivers and Predictive Biomarkers.
Article Snippet: Images were scanned using a Philips Digital scanner. .. FN1 knockdown with Migration and Invasion Assays Two human HCC cell lines SK-Hep1 and SNU182 were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA) and maintained in complete DMEM media (Gibco, USA) with 10% FBS in a 37C incubator. .. Scr siRNA (SR30004) and three unique FN1 siRNA duplexes (SR320193) were purchased from Origene, Inc. Trilencer-27 fluorescent labeled transfection control (SR30002) was first used to determine optimal transfection efficiency for each cell line.

Migration:

Cell Culture:

Article Title: Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis
Article Snippet: .. The human HCC cell lines SK-Hep1 and SUN-423 were obtained from the American Type Cell Culture Collection (ATCC), Hep3B and Huh7 were purchased from the Cell Bank of the Chinese Academy of Sciences, and SMMC-7721, MHCC-97 L and MHCC-LM3 were preserved in Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University. .. All HCC cell lines were cultivated in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Hyclone).

Article Title: IL-37 dampens immunosuppressive functions of MDSCs via metabolic reprogramming in the tumor microenvironment.
Article Snippet: All animal experimental procedures were conducted in accordance with the guidelines of Agri-Food and Veterinary Authority and the National Advisory Committee for Laboratory Animal Research of Singapore and were approved by the International Animal Care and Use Committee (IACUC), A*STAR (BRC #181367) and National University of Singapore (R19-1173). .. Murine HCC cell line Hepa1-6, human HCC cell lines Sk-Hep1, SMMC-7721 and human embryonic kidney line 293T cells were obtained from the American Type Culture Collection (Manassas, VA) and cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 100 IU/mL penicillin/streptomycin, 1 mM sodium pyruvate, 1 mM Cell Reports 43, 113835, March 26, 2024 21 nonessential amino acids and 10 mM HEPES at 37 C, 5% CO2 (Gibco, Carlsbad, CA). ..

Article Title: Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis.
Article Snippet: .. The human HCC cell lines SK-Hep1 and SUN-423 were obtained from the American Type Cell Culture Collection (ATCC), Hep3B and Huh7 were purchased from the Cell Bank of the Chinese Academy of Sciences, and SMMC-7721, MHCC-97 L and MHCC-LM3 were preserved in Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University. .. All HCC cell lines were cultivated in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Hyclone).

Modification:

Planar Chromatography:

Article Title: Dysregulated glucuronic acid metabolism exacerbates hepatocellular carcinoma progression and metastasis through the TGFβ signalling pathway
Article Snippet: .. Human HCC cell lines SK‐Hep1, SNU449, HepG2 and PLC/PRF/5 were obtained from the American Type Culture Collection (VA, USA); Hep3B, Huh7, HEK293T from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). ..

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